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A HPLC separation with a Kinetex EVO C18 packed column in 11 min was optimized.
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Compared to our previously reported method [1], it offers alternatives with improvements in recoveries, number of analytes, sample volume and solvent used.
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Analysis of pharmaceuticals in fish using ultrasound extraction and dispersive spe clean

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Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c to before being QC accepted as a Peptide separation column.
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The upper part of the MS spectra corresponds to the experimental acquisition, while the lower part of the MS spectra corresponds to the database library.
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